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pPB_CAG-PE2-P2A-emiRFP670_hPGK-BSD
(Plasmid #253553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253553 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Unspecified
  • Backbone manufacturer
    VectorBuilder
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PE2
  • Species
    Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.16.613361 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB_CAG-PE2-P2A-emiRFP670_hPGK-BSD was a gift from Ophir Shalem (Addgene plasmid # 253553 ; http://n2t.net/addgene:253553 ; RRID:Addgene_253553)

  • For your References section:

    High-throughput optimized prime editing mediated endogenous protein tagging for pooled imaging of protein localization. Sanchez HM, Lapidot T, Shalem O. bioRxiv [Preprint]. 2024 Sep 17:2024.09.16.613361. doi: 10.1101/2024.09.16.613361. 10.1101/2024.09.16.613361 PubMed 39345511
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