High-throughput optimized prime editing mediated endogenous protein tagging for pooled imaging of protein localization.
Sanchez HM, Lapidot T, Shalem O
bioRxiv [Preprint]. 2024 Sep 17:2024.09.16.613361. doi: 10.1101/2024.09.16.613361.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253553 | pPB_CAG-PE2-P2A-emiRFP670_hPGK-BSD | Prime editing in mammalian cells using PE2 |
| 253554 | pCMV_mNG211-Clathrin light chain | Express mNG2(11) for use of split mNeonGreen |
| 253555 | pLenti_pegRNA-H2BC21-Cterm-NG11-48bp-Puro | Express pegRNA to tag C-term of H2BC21 with mNG11, used as lenti |
| 253556 | CROPseq-pegRNA-BC-acceptor-Puro | pegRNA acceptor plasmid for library generation |
| 253557 | pSB-pegRNA-acceptor-Puro | pegRNA acceptor plasmid for sleeping beauty integration |
| 253558 | pSB-pegRNA-BUD23-Nterm-NG11-60bp-Puro | pegRNA to efficiently tag N-term of BUD23 with mNG11, integrated through Sleeping Beauty transposase |
| 253559 | pSB-pegRNA-CDK4-Cterm-NG11-60bp-Puro | pegRNA to efficiently tag C-term of CDK4 with mNG11, integrated through Sleeping Beauty transposase |
| 253560 | pSB-pegRNA-COPG1-Cterm-NG11-60bp-Puro | pegRNA to efficiently tag C-term of COPG1 with mNG11, integrated through Sleeping Beauty transposase |
| 253561 | pSB-pegRNA-ESYT1-Nterm-NG11-60bp-Puro | pegRNA to efficiently tag N-term of ESYT1 with mNG11, integrated through Sleeping Beauty transposase |
| 253562 | pSB-pegRNA-NUFIP2-Cterm-NG11-60bp-Puro | pegRNA to efficiently tag C-term of NUFIP2 with mNG11, integrated through Sleeping Beauty transposase |
| 253563 | pSB-pegRNA-TRIM28-Nterm-NG11-60bp-Puro | pegRNA to efficiently tag N-term of TRIM28 with mNG11, integrated through Sleeping Beauty transposase |