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pLenti_pegRNA-H2BC21-Cterm-NG11-48bp-Puro
(Plasmid #253555)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253555 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    CROPseq-puro-v2 (Addgene #127458)
  • Backbone manufacturer
    Paul Blainey
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pegRNA: H2BC21, insert mNG11
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Synthetic
  • Promoter mU6

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Removed all BbsI sites.

Please visit https://doi.org/10.1101/2024.09.16.613361 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_pegRNA-H2BC21-Cterm-NG11-48bp-Puro was a gift from Ophir Shalem (Addgene plasmid # 253555 ; http://n2t.net/addgene:253555 ; RRID:Addgene_253555)

  • For your References section:

    High-throughput optimized prime editing mediated endogenous protein tagging for pooled imaging of protein localization. Sanchez HM, Lapidot T, Shalem O. bioRxiv [Preprint]. 2024 Sep 17:2024.09.16.613361. doi: 10.1101/2024.09.16.613361. 10.1101/2024.09.16.613361 PubMed 39345511
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