pHLP728
(Plasmid #253586)

• Purpose: Integration of cassette for chromosome tagging of C. albicans target genes at 3' end with AID*-3HA degron and expression of OsTIR1(F74A)-3Myc from ACT1 promoter with SAT1 (nourseothricin) selection
• Depositing Lab: Mark Hall
• Depositing Organization: Purdue University
  Located in United States of America
• Publication: Hall Lab Plasmids Feb 2026 (unpublished)

Backbone

• Vector backbone: pBluescript II SK+
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AID*-3HA and OsTIR1(F74A)-3Myc
• Species: Oryza sativa
• Mutation: F74A variant of Oryza sativa TIR1 gene

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry