pHLP728
(Plasmid
#253586)
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PurposeIntegration of cassette for chromosome tagging of C. albicans target genes at 3' end with AID*-3HA degron and expression of OsTIR1(F74A)-3Myc from ACT1 promoter with SAT1 (nourseothricin) selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBluescript II SK+
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAID*-3HA and OsTIR1(F74A)-3Myc
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SpeciesOryza sativa
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MutationF74A variant of Oryza sativa TIR1 gene
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHLP728 was a gift from Mark Hall (Addgene plasmid # 253586 ; http://n2t.net/addgene:253586 ; RRID:Addgene_253586)