pHLP908
(Plasmid
#253593)
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PurposeChromosomal tagging of C. albicans target genes at 5' end with 3V5-AID* degron sequence using recyclable CaKAN (G418) selection marker in AID2 system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBluescript II SK+
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3V5-AID*
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SpeciesUnspecified
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains the natural promoter region of PPH21 from C. albicans. For N-terminal tagging of other targets, replace the PPH21 promoter region with the natural promoter of the desired target gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHLP908 was a gift from Mark Hall (Addgene plasmid # 253593 ; http://n2t.net/addgene:253593 ; RRID:Addgene_253593)