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pNos-myrGFP
(Plasmid #253595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253595 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    nanos>MCP-GFP
  • Backbone manufacturer
    Takashi Fukaya
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 8990
  • Modifications to backbone
    BamHI, XhoI digestion
  • Vector type
    Insect Expression
  • Selectable markers
    mini-white (Drosophila eye color marker)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myrGFP
  • Alt name
    myristoylated green fluorescent protein
  • Species
    D. melanogaster (fly), Synthetic
  • Insert Size (bp)
    978
  • Promoter Nanos promoter
  • Tag / Fusion Protein
    • myristoylation signal (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Nos-myrGFP-F: 5’-CCCGGATTTTCGCCCTCGAGATGGGCAACAAATGCTGCAG-3’
  • 3′ sequencing primer Nos-myrGFP-R: 5’-AAGTGGGCGTGACGCGGATCCTTACTTGTAGAGCTCATCCA-3’
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gerald Rubin, pJFRC12-10XUAS-IVS-myr::GFP (Addgene Plasmid # 26222)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNos-myrGFP was a gift from Mayu Inaba-Oguro (Addgene plasmid # 253595 ; http://n2t.net/addgene:253595 ; RRID:Addgene_253595)
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