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pSGAb-gfp
(Plasmid #253605)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253605 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSGAb
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6073
  • Total vector size (bp) 6797
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Green fluorescent protein
  • Alt name
    GFP
  • Species
    Aequoria victoria
  • Insert Size (bp)
    717
  • GenBank ID
    LN515608.1 LN515608.1
  • Promoter J23119(SpeI) promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGGATCCCGCCatgagtaaaggagaagaacttttc
  • 3′ sequencing primer GCTCTAGAGCtggacatttatttgtatagttcatccatgc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSGAb-gfp was a gift from Woojun Park (Addgene plasmid # 253605 ; http://n2t.net/addgene:253605 ; RRID:Addgene_253605)
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