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pSGAb-mCherry
(Plasmid #253606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253606 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSGAb
  • Backbone size w/o insert (bp) 6073
  • Total vector size (bp) 6783
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    monomeric derivative of DsRed fluorescent protein
  • Alt name
    mCherry
  • Species
    Discosoma (a marine coral)
  • Insert Size (bp)
    711
  • GenBank ID
    MF169983.1 MF169983.1
  • Promoter J23119(SpeI) promoter
  • Tag / Fusion Protein
    • tag (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGGATCCCGCCatggtgagcaagggcgaggaggat
  • 3′ sequencing primer GCTCTAGAGctacttgtacagctcgtccatgc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, finding x2 mCherry. The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSGAb-mCherry was a gift from Woojun Park (Addgene plasmid # 253606 ; http://n2t.net/addgene:253606 ; RRID:Addgene_253606)
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