mNeonAce-unc45
(Plasmid
#253619)
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PurposeZebrafish skeletal muscle/adaxial expression of Ace2N-mNeon for voltage imaging
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDestTol pA2
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAce2N-mNeon
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SpeciesD. rerio (zebrafish); Acetabularia acetabulum; Branchiostoma lanceolatum
- Promoter unc45
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byReceived from Adam Cohen (Harvard University), at Delft University of Technology
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mNeonAce-unc45 was a gift from Daan Brinks (Addgene plasmid # 253619 ; http://n2t.net/addgene:253619 ; RRID:Addgene_253619) -
For your References section:
Zebrafish Ace2N-mNeon expression toolkit for in vivo voltage imaging of neuronal synchrony and cardiac maturation. Wu Z, Oliveira Silva R, Houssein R, Marques Trujillo F, Gotti J, Ganapathy S, Gao Z, Brinks D. Neurophotonics. 2026 Feb;13(Suppl 2):S23205. doi: 10.1117/1.NPh.13.S2.S23205. Epub 2026 May 9. 10.1117/1.NPh.13.S2.S23205 PubMed 42109300