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NCMV HPV101 E7
(Plasmid #253678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253678 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pNCMV
  • Backbone manufacturer
    Karl Munger (Addgene #37453)
  • Backbone size w/o insert (bp) 6548
  • Total vector size (bp) 6848
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV101 E7
  • Alt name
    HPV101_gp1
  • Species
    HPV101
  • Insert Size (bp)
    296
  • GenBank ID
    NC_008189.1
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag/HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer CMV bam neo 5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NCMV HPV101 E7 was a gift from Karl Munger (Addgene plasmid # 253678 ; http://n2t.net/addgene:253678 ; RRID:Addgene_253678)

  • For your References section:

    The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7. Gelbard MK, Grace M, von Schoeler-Ames A, Gnanou I, Munger K. Tumour Virus Res. 2025 Jun;19:200300. doi: 10.1016/j.tvr.2024.200300. Epub 2024 Dec 5. 10.1016/j.tvr.2024.200300 PubMed 39643241
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