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pcDNA3.1-HA-H3N2-mTagBFP2
(Plasmid #253734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253734 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/Thermo Fisher Scientific
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA (A/Hong Kong/4801/2014, H3N2)
  • Species
    Influenza A virus
  • Promoter CMV
  • Tag / Fusion Protein
    • mTagBFP2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon-optimized gene fragment synthesized by Twist Bioscience

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon-optimized influenza A hemagglutinin A/Hong Kong/4801/2014 (H3N2), including endogenous signal peptide and lacking stop codon, fused C-terminally via a 7xGS linker to mTagBFP2 in the pcDNA3.1 backbone. Plasmid used in the multiplex HA antibody binding assay described in Daniel et al., 2026. A detailed step-by-step protocol will be available in a forthcoming STAR Protocols publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HA-H3N2-mTagBFP2 was a gift from Christoph Kreer (Addgene plasmid # 253734 ; http://n2t.net/addgene:253734 ; RRID:Addgene_253734)

  • For your References section:

    Pre-existing neutralizing antibodies against cattle-transmitted influenza A virus H5N1 are detectable in unexposed individuals. Daniel K, Ullrich L, Ruchnewitz D, Meijers M, Halwe NJ, Wild U, Eberhardt J, Schon J, Stumpf R, Schlotz M, Wunsch M, Girao Lessa L, Abdelwhab EM, Kuryshko M, Dietrich C, Pinger A, Schumacher AL, Germer M, Rohde M, Kukat C, Gieselmann L, Gruell H, Hoffmann D, Beer M, Erren T, Lassig M, Kreer C, Klein F. Immunity. 2026 Feb 10;59(2):494-508.e8. doi: 10.1016/j.immuni.2025.12.013. Epub 2026 Jan 30. 10.1016/j.immuni.2025.12.013 PubMed 41619731
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