pIRES2-EGFP-hEAG1-Variant 1
(Plasmid #253763)

• Purpose: Plasmid for expression of the wildtype human EAG1/KCNH1, isoform 1 with an IRES-EGFP serving as reporter.
• Depositing Lab: Al George
• Publication: George Lab EAG1 Plasmids 2026 (unpublished)

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EAG1
• Species: H. sapiens (human)
• Insert Size (bp): 2970
• Mutation: c.T894C
• Entrez Gene: KCNH1 (a.k.a. EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Roland Schoenherr, https://pubmed.ncbi.nlm.nih.gov/10880439/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a G > A transition at nuctiotide 480 of the EMCV IRES and a C > T transition at nucleotide 148 of the replication origin (bps 1086 and 4693 respectively relative to parent plasmid map). The hEAG1 ORF contains a intentional synonymous T > C transition at nucleotide 894 to address sequence instability issues in the pIRES2 backbone in E. coli.