pYY152-HO_Nterm_int-TP901-GFPdropout
(Plasmid
#253830)
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PurposePlasmid used for constructing donor DNA for TP901-assisted integration onto the yeast genome at the HO locus for N terminal display.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253830 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPink
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Vector typeDonor plasmid
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesfGFP
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a minor discrepancy between the Addgene NGS sequence and the depositor sequence: SNP in sfGFP Q80K. Depositor confirms this is not of concern for plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYY152-HO_Nterm_int-TP901-GFPdropout was a gift from Chang Liu (Addgene plasmid # 253830 ; http://n2t.net/addgene:253830 ; RRID:Addgene_253830)