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pACYC-NtHis6Ubq-RbcS
(Plasmid #253866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253866 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pACYC
  • Backbone size w/o insert (bp) 4783
  • Total vector size (bp) 5443
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tobacco Rubisco small subunit
  • Alt name
    NtRbcS
  • Species
    Nicotiana tabacum
  • Insert Size (bp)
    372
  • GenBank ID
    P69249.1
  • Entrez Gene
    LOC107802883 (a.k.a. rbcS)
  • Promoter trc
  • Tag / Fusion Protein
    • Histidine-ubiquitin (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid is prone to recombination. Please screen multiple clones for the full-length plasmid before proceeding.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYC-NtHis6Ubq-RbcS was a gift from Matthew D. Shoulders (Addgene plasmid # 253866 ; http://n2t.net/addgene:253866 ; RRID:Addgene_253866)
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