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pAAV-TRE-HA-hAAK1-Ser624Asp
(Plasmid #254003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254003 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV-TRE-mTurquoise2
  • Modifications to backbone
    Turquoise 2 is replaced with AAK1
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP2-associated protein kinase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2880
  • Mutation
    Serine 624 changed to aspartate
  • GenBank ID
    NM_014911.5
  • Entrez Gene
    AAK1
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer tcaagtgccacctgacgtc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was synthesized commercially

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-HA-hAAK1-Ser624Asp was a gift from Eleanor Coffey (Addgene plasmid # 254003 ; http://n2t.net/addgene:254003 ; RRID:Addgene_254003)
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