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pET21_Dengue-NS2B-NS3_Gibbs_GTM_001
(Plasmid #254043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254043 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET21_NESG
  • Backbone manufacturer
    Novagen backbone modified in the lab of Gaetano Montelione
  • Backbone size w/o insert (bp) 5444
  • Total vector size (bp) 6167
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Utilize BL21DE3 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NS2B-NS3 fusion
  • Species
    Dengue Virus
  • Insert Size (bp)
    723
  • Mutation
    GS Linker between NS2B and NS3
  • GenBank ID
    P14340 POLG_DEN2N AKR53696.1
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis-GlySer Linker-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21_Dengue-NS2B-NS3_Gibbs_GTM_001 was a gift from Gaetano Montelione (Addgene plasmid # 254043 ; http://n2t.net/addgene:254043 ; RRID:Addgene_254043)
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