pEV2
(Plasmid
#254053)
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Purpose(Empty Backbone) Genomic insertion vector for expression of inserts under bt1311 promoter. Constitutive mCherry expression. Derived from pWW3515.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepWW3515
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Backbone manufacturerJustin Sonnenburg Lab. Whitaker et al., Cell, 2017. DOI: 10.1016/j.cell.2017.03.041
- Backbone size (bp) 5855
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Modifications to backbonerpoD promoter (gene bt1311 from Bacteroides thetaiotaomicron) was inserted in BamHI-digested pWW3515 using Gibson Assembly. An XbaI site was left downstream of the rpoD promoter for insert cloning purposes.
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Vector typeBacterial Expression
- Promoter rpoD (for inserts), phage promoter (for mCherry in pWW3452)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsContains ermG gene for erythromycin selection in Bacteroides recipients.
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Publication for pWW3515 backbone: DOI: 10.1016/j.cell.2017.03.041.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEV2 was a gift from Juliane Bubeck-Wardenburg (Addgene plasmid # 254053 ; http://n2t.net/addgene:254053 ; RRID:Addgene_254053)
Map uploaded by the depositor.
