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pKan
(Plasmid #254147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254147 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTZ18R
  • Backbone manufacturer
    Pharmacia
  • Backbone size (bp) 2864
  • Modifications to backbone
    insertion of kanMX G418-resistance cassette and B form of S, cerevisiae 2-micron plasmid. Note FLP gene is disrupted, plasmid partitions but is amplification-incompetent in yeast
  • Vector type
    yeast-E.coli shuttle plasmid
  • Selectable markers
    kanMX - select with G418 in yeast, kanamycin in E.coli
  • Tag / Fusion Protein
    • None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKan was a gift from Melanie Dobson (Addgene plasmid # 254147 ; http://n2t.net/addgene:254147 ; RRID:Addgene_254147)

  • For your References section:

    pSB3, a member of the 2-micron family of plasmids, replicates and is efficiently partitioned in multiple budding yeast species. Mereshchuk A, Chew JSK, Dobson MJ. G3 (Bethesda). 2026 Feb 18:jkag012. doi: 10.1093/g3journal/jkag012. 10.1093/g3journal/jkag012 PubMed 41706541
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