Skip to main content
Addgene
Open Menu

pAAV-hSyn1EmGFP-3x-miR-shRNA(Cacna1b)-WPRE-bGHpA
(Plasmid #254157)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254157 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pAAV-hSyn1-EmGFP
  • Vector type
    Mammalian Expression, AAV, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cacna1b
  • Alt name
    Cav2.2
  • gRNA/shRNA sequence
    Cacna1b_1: CCTTACTTTCGGGATCTTT; Cacna1b_2: GCGCATCATACAATGACAT; Cacna1b_3: GCCGTAATATCATGGGATT
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cacna1b (a.k.a. BIII, Cav2.2, Cchn1a, alpha(1B))
  • Promoter hSyn1
  • Tag / Fusion Protein
    • EmGFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn1EmGFP-3x-miR-shRNA(Cacna1b)-WPRE-bGHpA was a gift from Hiromasa Funato (Addgene plasmid # 254157 ; http://n2t.net/addgene:254157 ; RRID:Addgene_254157)

  • For your References section:

    Early-life maternal care is required for the typical development of calming responses to back stroking. Yoshida S, Harauma A, Moriguchi T, Tsuneoka Y, Narikiyo K, Miyanishi K, Kashima M, Wada M, Hayashi Y, Funato H. Commun Biol. 2026 Apr 10;9(1):894. doi: 10.1038/s42003-026-10012-6. 10.1038/s42003-026-10012-6 PubMed 41963499
Related items: