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C321.ΔA [pFrec.ΔTAG]
(Bacterial strain #254204)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 254204 Bacteria in agar stab 1 $94
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Backbone

  • Vector backbone
    n/a
  • Vector type
    This is a strain, not a plasmid
  • Selectable markers
    kanR (F plasmid)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    C321.ΔA [pFrec.ΔTAG]
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MG1655.ΔTAG.ΔprfA.bioAB.Δλred [pFrec.ΔTAG]
  • Species
    n/a

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    “COMPOSITIONS AND METHODS FOR BIOCONTAINMENT OF MICROORGANISMS.”

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TAG-recoded E. coli with compatible TAG-recoded F plasmid for bacterial F-pilus expression within TAG-recoded GRO C321 (used in C321 phage experiments).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.ΔA [pFrec.ΔTAG] was a gift from Farren Isaacs (Addgene plasmid # 254204)

  • For your References section:

    Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements. Ma NJ, Isaacs FJ. Cell Syst. 2016 Aug;3(2):199-207. doi: 10.1016/j.cels.2016.06.009. Epub 2016 Jul 14. 10.1016/j.cels.2016.06.009 PubMed 27426981
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