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pKRB156
(Plasmid #254233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254233 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMV361
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    iRFP670
  • Species
    Rhodospirillum centenum
  • Promoter hsp60

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ctaagaataacgttggcactcgcgac
  • 3′ sequencing primer TGACACTCATTTCTTTCTCCTTAGGTCGACttagcgttggtggtgggcggcggtgaag
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    HoI
  • Alt name
    heme oxygenase 1
  • Species
    Synechocystis sp. PCC6803
  • Promoter hsp60

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GTCGACCTAAGGAGAAAGAAATGAGTGTCAACTTAGCTTCCCAGTTGCG
  • 3′ sequencing primer GAGACACAACGTCGCTTTGTTGGCTAGC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKRB156 was a gift from Manabu Ato (Addgene plasmid # 254233 ; http://n2t.net/addgene:254233 ; RRID:Addgene_254233)

  • For your References section:

    In vivo imaging identified efficient antimicrobial treatment against Mycobacterium marinum infection in mouse footpads. Yamamoto K, Torigoe S, Tsujimura Y, Asaka MN, Okumura K, Ato M. Sci Rep. 2024 Oct 17;14(1):24343. doi: 10.1038/s41598-024-75207-5. 10.1038/s41598-024-75207-5 PubMed 39420066
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