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258/-2.5lacZ
(Plasmid #25429)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25429 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 25429-D50 50 μg of DNA in Tris buffer $495
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DNA 25429-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pPD46.21
  • Backbone manufacturer
    Fire et al., 1990
  • Backbone size w/o insert (bp) 6110
  • Vector type
    Mammalian Expression ; Reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myo D Promoter
  • Alt name
    MyoD
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYOD1 (a.k.a. CMYO17, CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Tag / Fusion Protein
    • lacZ (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer n/A
  • 3′ sequencing primer LacZ-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site.

258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    258/-2.5lacZ was a gift from Charles P. Emerson Jr. (Addgene plasmid # 25429 ; http://n2t.net/addgene:25429 ; RRID:Addgene_25429)

  • For your References section:

    Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Goldhamer DJ, Brunk BP, Faerman A, King A, Shani M, Emerson CP. Development. 1995 Mar . 121(3):637-49. PubMed 7720572
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