Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #13494)


Item Catalog # Description Quantity Price (USD)
Plasmid 13494 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    Morgenstern&Land (NAR 18:3587); Available at Addgene
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    MyoD1-Estrogen Receptor Fusion Protein
  • Alt name
    Mouse Myod1
  • Alt name
    Human Estrogen receptor 1 alpha
  • Alt name
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    full length MyoD was fused to aa282-595 of Esr1. Esr1 is inserted between codons 173 and 174 of MyoD.
  • GenBank ID
  • Entrez Gene
    Myod1 (a.k.a. MYF3, MyoD, Myod-1, bHLHc1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Harold Weintraub Lab, Fred Hutchinson Cancer Research Center
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Entrez ID for MyoD is NM_010866 ; the Entrez ID for ER is NM_000125.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE MDER was a gift from Stephen Tapscott (Addgene plasmid # 13494 ; ; RRID:Addgene_13494)
  • For your References section:

    Use of a conditional MyoD transcription factor in studies of MyoD trans-activation and muscle determination. Hollenberg SM, Cheng PF, Weintraub H. Proc Natl Acad Sci U S A. 1993 Sep 1. 90(17):8028-32. 10.1073/pnas.90.17.8028 PubMed 8396258