pEPCT1SP0177
(Plasmid
#254305)
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PurposepTRV2:CoPDS:GFP:FT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTRV2-GG
- Backbone size w/o insert (bp) 11305
- Total vector size (bp) 10539
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Vector typeVIGS
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDS:GFP:FT
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SpeciesCalendula officinalis
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Insert Size (bp)697
- Promoter CaMV35S
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer caaggaagcacgatgag
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPCT1SP0177 was a gift from Nicola Patron (Addgene plasmid # 254305 ; http://n2t.net/addgene:254305 ; RRID:Addgene_254305)