mStay Gold Nat
(Plasmid
#254395)
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PurposePlasmid for tagging C-terminal yeast proteins with mStay Gold with NatR marker, also has a 3xHA tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAG25
- Backbone size w/o insert (bp) 3704
- Total vector size (bp) 4358
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Vector typeYeast Expression
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemStayGold
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SpeciesCytaeis uchidae
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Insert Size (bp)654
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer cgatttaggtgacactat
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mStay Gold Nat was a gift from Aaron Hoskins (Addgene plasmid # 254395 ; http://n2t.net/addgene:254395 ; RRID:Addgene_254395)