p11 SNAPv2
(Plasmid
#254400)
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PurposeSNAPv2 gene inserted via NdeI & BamHI cut sites into p11 backbone for protein expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonep11
- Backbone size w/o insert (bp) 5680
- Total vector size (bp) 6199
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP tag-2
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Insert Size (bp)519
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p11 SNAPv2 was a gift from Aaron Hoskins (Addgene plasmid # 254400 ; http://n2t.net/addgene:254400 ; RRID:Addgene_254400)
Map uploaded by the depositor.
