pLPC-S22D-LaminA-CS
(Plasmid #254401)

• Purpose: Encodes a mutant LaminA-CS where serine 22 is mutated to aspartic acid
• Depositing Lab: Gerardo Ferbeyre
• Publication: LaminA S22D mutants (unpublished)

Backbone

• Vector backbone: pLPC
• Backbone size w/o insert (bp): 5629
• Total vector size (bp): 7624
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LMNA
• Species: H. sapiens (human)
• Insert Size (bp): 1995
• Mutation: S22D: serine 22 mutated to aspartic acid; CS: cysteine 661 mutated to serine
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: CMV

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: taggcgtgtacggtggga
• 3′ sequencing primer: acctgtaggtttggcaag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry