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336_pETcon_SARS2_KP.3.1
(Plasmid #254410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254410 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pETcon
  • Backbone manufacturer
    Andrew Scharenberg (Addgene #41522
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 KP.3.1 RBD
  • Species
    S. cerevisiae (budding yeast); SARS-CoV-2 virus
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • c-Myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.03.11.711006 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    336_pETcon_SARS2_KP.3.1 was a gift from Tyler Starr (Addgene plasmid # 254410 ; http://n2t.net/addgene:254410 ; RRID:Addgene_254410)

  • For your References section:

    Deep mutational scanning of recent SARS-CoV-2 variants highlights changing amino acid preferences within epistatic hotspot residues. Taylor AL, Starr TN. PLoS Pathog. 2026 Jun 22;22(6):e1014074. doi: 10.1371/journal.ppat.1014074. eCollection 2026 Jun. 10.1371/journal.ppat.1014074 PubMed 42330076
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