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AAV-Gfa104-jGCaMP8f
(Plasmid #254431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254431 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    AAV2 packaging
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fast calcium green fluorescent indicator
  • Alt name
    jGCaMP8f
  • Species
    Synthetic
  • Promoter Gfa104

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gfa104 promoter is astrocyte specific; jGCaMP8f is fast green detector of intracellular Ca2+ for multi-photon

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Gfa104-jGCaMP8f was a gift from Christopher Norris (Addgene plasmid # 254431 ; http://n2t.net/addgene:254431 ; RRID:Addgene_254431)

  • For your References section:

    Disrupted Calcium Dynamics in Reactive Astrocytes Occur with End Feet-Arteriole Decoupling in an Amyloid Mouse Model of Alzheimer's Disease. Weiss BE, Gant JC, Lin RL, Gollihue JL, Rogers CB, Kraner SD, Rucker EB, Katsumata Y, Jiang Y, Nelson PT, Wilcock DM, Sompol P, Thibault O, Norris CM. J Neurosci. 2025 Oct 1;45(40):e0349252025. doi: 10.1523/JNEUROSCI.0349-25.2025. 10.1523/JNEUROSCI.0349-25.2025 PubMed 40816764
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