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TP1453
(Plasmid #254449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254449 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    #135448
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 10325
  • Total vector size (bp) 11657
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-EMC inhibitor nanobody C10
  • Species
    Alpaca (Vicugna pacos)
  • Mutation
    Contains engineered nanobody variant with ALFA tag.
  • Promoter EF1α

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TP1453 was a gift from Tino Pleiner (Addgene plasmid # 254449 ; http://n2t.net/addgene:254449 ; RRID:Addgene_254449)

  • For your References section:

    The ER membrane protein complex acts as a chaperone to promote the biogenesis of multi-bundle membrane proteins. Stanton M, Singal B, Biswal M, Agarwal M, Scheuing CE, Vargas GD, Gao A, Gifford CA, Pleiner T. bioRxiv [Preprint]. 2026 Jan 15:2026.01.14.699575. doi: 10.64898/2026.01.14.699575. 10.64898/2026.01.14.699575 PubMed 41648177
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