iAAV-TnnT2-Nrf1
(Plasmid
#254459)
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PurposeExpresses Nrf1 (encoded by Nfe2l1) upon Dox induction specifically in TnT2 promoter active cardiomyocytes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneAAV9
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNrf1 (encoded by nfe2l1)
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SpeciesM. musculus (mouse)
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Entrez GeneNfe2l1 (a.k.a. LCR-F1, Lcrf1, NRF1, TCF-11, TCF11)
- Promoter TRE
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ccctcgtaaagaattcGCCGCCACCATGCTTTCTCTGAAGAAATATTTAACGG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iAAV-TnnT2-Nrf1 was a gift from Miao Cui (Addgene plasmid # 254459 ; http://n2t.net/addgene:254459 ; RRID:Addgene_254459)