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pGOLM1-EGFP
(Plasmid #254490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254490 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) -1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GOLM1
  • Alt name
    GOLM1
  • Species
    H. sapiens (human)
  • Entrez Gene
    GOLM1 (a.k.a. C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-f primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGOLM1-EGFP was a gift from Dominik Buser (Addgene plasmid # 254490 ; http://n2t.net/addgene:254490 ; RRID:Addgene_254490)

  • For your References section:

    Golgi enzymes are retrieved from the plasma membrane to the trans-Golgi network. Buser DP, Junne T. FEBS Lett. 2026 Aug 15. doi: 10.1002/1873-3468.70438. 10.1002/1873-3468.70438 PubMed 42603102
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