pQQ95
(Plasmid
#254494)
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PurposeNLP7 nitrate sensor built based on pQQ7, pQQ90, and pQQ132
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Depositing Lab
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Depositing OrganizationCalifornia Institute of Technology
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCsC
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCsC_F_pOlesin_FASTred+F_p4×[NLP7-TATA]_pMinDFR_GeNL[ST-LS1 intron2_v3]_THsp18+R_pNOS_NLuc[ST-LS1 intron2_v3]_Tnos
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SpeciesA. thaliana (mustard weed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.03.18.712767 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQQ95 was a gift from Gözde Demirer (Addgene plasmid # 254494 ; http://n2t.net/addgene:254494 ; RRID:Addgene_254494) -
For your References section:
Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments. Li E, Berruto C, Oz TM, Grillo E, Griffin C, Wang Y, Muchenje KT, Jones JW, Demirer GS. Proc Natl Acad Sci U S A. 2026 Jul 28;123(30):e2609666123. doi: 10.1073/pnas.2609666123. Epub 2026 Jul 23. 10.1073/pnas.2609666123 PubMed 42490377