|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25451||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2500
Vector typePichia pastoris expression
Growth in Bacteria
Gene/Insert namePichia pastoris HIS4
Insert Size (bp)2532
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIB2 was a gift from Benjamin Glick (Addgene plasmid # 25451 ; http://n2t.net/addgene:25451 ; RRID:Addgene_25451)
For your References section:A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Sears IB, O'Connor J, Rossanese OW, Glick BS. Yeast. 1998 Jun 15;14(8):783-90. 10.1002/(SICI)1097-0061(19980615)14:8<783::AID-YEA272>3.0.CO;2-Y PubMed 9675822