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pTBX1-αMoonTag-Tn5
(Plasmid #254522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254522 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    3XFlag-pA-Tn5-Fl
  • Backbone manufacturer
    Steven Henikoff (Addgene plasmid # 124601)
  • Backbone size w/o insert (bp) 6654
  • Total vector size (bp) 8562
  • Modifications to backbone
    Removal of 3xFLAG and Protein A. Replaced with anti-MoonTag nanobody in-frame with Tn5
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-MoonTag::Tn5
  • Species
    Synthetic
  • Insert Size (bp)
    1908
  • Tags / Fusion Proteins
    • Mxe GyrA intein (C terminal on backbone)
    • Chitin Binding Domain (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.04.27.721191 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTBX1-αMoonTag-Tn5 was a gift from Derek Janssens (Addgene plasmid # 254522 ; http://n2t.net/addgene:254522 ; RRID:Addgene_254522)

  • For your References section:

    CoCUT&Tag maps linked chromatin states at single-molecule, single-cell resolution. Sun W, Baird E, Ashbaugh HJ, Eiken AP, Janssens DH. bioRxiv 2026.04.27.721191 10.64898/2026.04.27.721191
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