pBAD02-BcNhi-like
(Plasmid
#254553)
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PurposeFor the expression of the Nhi-like system encoded by Bacillus cereus FSL H8-0482
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD02
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcNhi
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Alt nameIMG 2729017301
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SpeciesBacillus cereus FSL H8-0482
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byGene insert received from Rotem Sorek, Weizmann Institute (PMID: 36302390)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A Q41R mutation in the insert was found; this does not affect function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD02-BcNhi-like was a gift from Philip Kranzusch (Addgene plasmid # 254553 ; http://n2t.net/addgene:254553 ; RRID:Addgene_254553)