pBAD02-EcMokosh type II
(Plasmid
#254563)
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PurposeFor the expression of the Mokosh type II system encoded by Escherichia coli 5-366-08_S1_C1
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD02
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEcMkoC
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Alt nameWP_000344089.1
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SpeciesEscherichia coli 5-366-08_S1_C1
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byGene insert received from Rotem Sorek, Weizmann Institute (PMID: 36302390)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A P324L mutation in the insert was found; this does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD02-EcMokosh type II was a gift from Philip Kranzusch (Addgene plasmid # 254563 ; http://n2t.net/addgene:254563 ; RRID:Addgene_254563)