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pLenti-CRISPRv2-sgEnpp1_#2-hygro
(Plasmid #254675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254675 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLenti-CRISPRv2
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA with Cas9 and hygromycin resistance
  • gRNA/shRNA sequence
    ATATGACTGTACCCTACGGG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Enpp1 (a.k.a. 4833416E15Rik, CD203c, E-NPP 1, E-NPP1, Ly-41, M6S1, NPP1, Npps, PC-1, Pca, Pca-1, Pdnp1, ttw, twy)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CRISPRv2-sgEnpp1_#2-hygro was a gift from Brian Ruffell (Addgene plasmid # 254675 ; http://n2t.net/addgene:254675 ; RRID:Addgene_254675)
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