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pCDB24-XhoI_bmTyr*
(Plasmid #254740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254740 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDB24
  • Backbone size w/o insert (bp) 7657
  • Total vector size (bp) 8527
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    bmTyr*
  • Species
    B. megaterium
  • Insert Size (bp)
    868
  • Tags / Fusion Proteins
    • HIS (N terminal on backbone)
    • SUMO (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDB24-XhoI_bmTyr* was a gift from Brian Kuhlman (Addgene plasmid # 254740 ; http://n2t.net/addgene:254740 ; RRID:Addgene_254740)

  • For your References section:

    Enhancing Enzymatic Bioconjugation Efficiency via Computer-Based Installation of a Substrate Recruitment Domain. Shelby C, Kuzelka KP, Ellis JM, Yao Z, McCue AC, Park R, Nair SK, Bowers A, Kuhlman B. Bioconjug Chem. 2026 Mar 17. doi: 10.1021/acs.bioconjchem.5c00468. 10.1021/acs.bioconjchem.5c00468 PubMed 41844172
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