pFBDMb DDB1 delta BPB + DDA1
(Plasmid
#254743)
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Purposerequired for Protein Expression DCAF 16 in Baculovirus Expression system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepFBDMb
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameDNA damage-binding protein 1
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SpeciesH. sapiens (human)
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Entrez GeneDDB1 (a.k.a. DDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF)
Gene/Insert 2
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Gene/Insert nameDET1- and DDB1-associated protein 1
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SpeciesH. sapiens (human)
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Entrez GeneDDA1 (a.k.a. C19orf58, PCIA1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFBDMb DDB1 delta BPB + DDA1 was a gift from Alessio Ciulli (Addgene plasmid # 254743 ; http://n2t.net/addgene:254743 ; RRID:Addgene_254743) -
For your References section:
Targeted protein degradation via intramolecular bivalent glues. Hsia O, Hinterndorfer M, Cowan AD, Iso K, Ishida T, Sundaramoorthy R, Nakasone MA, Imrichova H, Schatz C, Rukavina A, Husnjak K, Wegner M, Correa-Saez A, Craigon C, Casement R, Maniaci C, Testa A, Kaulich M, Dikic I, Winter GE, Ciulli A. Nature. 2024 Mar;627(8002):204-211. doi: 10.1038/s41586-024-07089-6. Epub 2024 Feb 21. 10.1038/s41586-024-07089-6 PubMed 38383787