Recombinant human CDK5RAP2 aa50-93
(Plasmid
#254744)
-
PurposeExpresses human CDK5RAP2 truncation in E.coli
-
Depositing Lab
-
Depositing OrganizationETH Zurich (Swiss Federal Institute of Technology)
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepET
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Recombinant human CDK5RAP2 aa50-93 was a gift from Michal Wieczorek (Addgene plasmid # 254744 ; http://n2t.net/addgene:254744 ; RRID:Addgene_254744) -
For your References section:
Partial closure of the gamma-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Xu Y, Munoz-Hernandez H, Krutyholowa R, Marxer F, Cetin F, Wieczorek M. Dev Cell. 2024 Dec 2;59(23):3161-3174.e15. doi: 10.1016/j.devcel.2024.09.002. Epub 2024 Sep 24. 10.1016/j.devcel.2024.09.002 PubMed 39321808