pFBDMb 6His_TEV DCAF16
(Plasmid
#254747)
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PurposepFBDMb_6His-TEV DCAF16 for Protein overexpression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepFBDMb
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDCAF16
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Alt nameDDB1- and CUL4-associated factor 16
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SpeciesH. sapiens (human)
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Entrez GeneDCAF16 (a.k.a. C4orf30)
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Tag
/ Fusion Protein
- 6x His TEV
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFBDMb 6His_TEV DCAF16 was a gift from Alessio Ciulli (Addgene plasmid # 254747 ; http://n2t.net/addgene:254747 ; RRID:Addgene_254747) -
For your References section:
Targeted protein degradation via intramolecular bivalent glues. Hsia O, Hinterndorfer M, Cowan AD, Iso K, Ishida T, Sundaramoorthy R, Nakasone MA, Imrichova H, Schatz C, Rukavina A, Husnjak K, Wegner M, Correa-Saez A, Craigon C, Casement R, Maniaci C, Testa A, Kaulich M, Dikic I, Winter GE, Ciulli A. Nature. 2024 Mar;627(8002):204-211. doi: 10.1038/s41586-024-07089-6. Epub 2024 Feb 21. 10.1038/s41586-024-07089-6 PubMed 38383787