Rit-APP12-YA3-HC
(Plasmid
#254750)
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PurposeRituximab HC N299Q
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAH
- Backbone size w/o insert (bp) 4562
- Total vector size (bp) 5909
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRituximab HC
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Alt nameRit-APP12-YA3-HC
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)1345
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MutationChanged Asparagine 299 to Glutamine
Resource Information
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A portion of this plasmid was derived from a plasmid made byTwist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rit-APP12-YA3-HC was a gift from Brian Kuhlman (Addgene plasmid # 254750 ; http://n2t.net/addgene:254750 ; RRID:Addgene_254750) -
For your References section:
Enhancing Enzymatic Bioconjugation Efficiency via Computer-Based Installation of a Substrate Recruitment Domain. Shelby C, Kuzelka KP, Ellis JM, Yao Z, McCue AC, Park R, Nair SK, Bowers A, Kuhlman B. Bioconjug Chem. 2026 Mar 17. doi: 10.1021/acs.bioconjchem.5c00468. 10.1021/acs.bioconjchem.5c00468 PubMed 41844172