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Tol2-LyzC-GFP-pa-u6ac-Gcat1-guides
(Plasmid #254767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254767 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Tol2-lyzc
  • Vector type
    CRISPR ; Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gcat1
  • gRNA/shRNA sequence
    GGTCATTCAGGTCCATGTGC, TGCTGGGCTGTAAAACACCC
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    gcat
  • Promoter Lyzc

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.08.652481 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-LyzC-GFP-pa-u6ac-Gcat1-guides was a gift from Qing Deng (Addgene plasmid # 254767 ; http://n2t.net/addgene:254767 ; RRID:Addgene_254767)

  • For your References section:

    RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization. Kim DH, Syahirah R, Zheng C, Ding C, Hsu AY, Pikes T, Liang Z, Liu S, Li L, Bao X, Umulis D, Wan J, Deng Q. J Leukoc Biol. 2026 Feb 9;118(2):qiaf174. doi: 10.1093/jleuko/qiaf174. 10.1093/jleuko/qiaf174 PubMed 41701563
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