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AiP14945 - pAAV-AiE0410m-minBG-iCre(R297T)-3XmiR126TS-BGHpA
(Plasmid #254824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254824 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 4662
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Growth at 30 degrees C in NEB Stable or Stbl3 cells recommended for pAAV backbone to minimize the likelihood of ITR rearrangements.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iCre
  • Species
    Synthetic
  • Mutation
    R297T for less recombinase activity which yields specificity with AAV delivery

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.20.558718 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AiP14945 - pAAV-AiE0410m-minBG-iCre(R297T)-3XmiR126TS-BGHpA was a gift from Allen Institute for Brain Science & Boaz Levi & Jonathan Ting (Addgene plasmid # 254824 ; http://n2t.net/addgene:254824 ; RRID:Addgene_254824)

  • For your References section:

    Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species. Mich JK, Sunil S, Johansen N, Martinez RA, Liu J, Gore BB, Mahoney JT, Leytze M, Ben-Simon Y, Bertagnolli D, Bhowmik R, Bishaw Y, Brouner K, Campos J, Canfield R, Casper T, Donadio NP, Dotson NI, Egdorf T, Gary A, Gibson S, Goldy J, Groce EL, Hagihara KM, Hirschstein D, Hou H, Laird WD, Liang E, Loftus LV, Lusk N, Malone J, Martin NX, Monet D, Nagra JS, Newman D, Ngo NK, Olsen P, Omstead V, Opitz-Araya X, Oster A, Pom CA, Potekhina L, Reding M, Rimorin C, Ruiz A, Sedeño-Cortés AE, Shapovalova NV, Taormina M, Taskin N, Tieu M, Valera Cuevas NJ, Way SW, Weed N, Wright V, Yao Z, Zhou T, McMillen DA, Kunst M, McGraw M, Thyagarajan B, Waters J, Bakken TE, Dee N, Yao S, Smith KA, Svoboda K, Podgorski K, Kojima Y, Horwitz GD, Zeng H, Daigle TL, Lein ES, Tasic B, Ting JT, Levi BP. eLife 14:RP108640 10.7554/eLife.108640.1
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