U1 promoter-terminator
(Plasmid
#254836)
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PurposeGuide-targeted RNA editing mediated by U1 snRNA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
- Total vector size (bp) 3121
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU1 snRNA Promoter-Terminator
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SpeciesH. sapiens (human)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
U1 promoter-terminator was a gift from Eugene Yeo (Addgene plasmid # 254836 ; http://n2t.net/addgene:254836 ; RRID:Addgene_254836) -
For your References section:
Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs. Smargon AA, Pant D, Gomberg TA, Fagre C, Glynne S, Nguyen J, Naritomi JT, Gilbert WV, Yeo GW. Nat Chem Biol. 2025 Sep 18:10.1038/s41589-025-02026-8. doi: 10.1038/s41589-025-02026-8. 10.1038/s41589-025-02026-8 PubMed 40968292