pAAV_EF1a>RPS2-APOBEC1-p2a-TdTomato-WPRE249-BGHpA
(Plasmid
#254839)
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PurposeRibo-STAMP AAV to detect translation dynamics
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254839 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 7068
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPS2
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SpeciesH. sapiens (human)
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Insert Size (bp)879
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GenBank IDNC_000016.10
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Entrez GeneRPS2 (a.k.a. LLREP3, S2, uS5)
- Promoter EF-1 alpha
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Tag
/ Fusion Protein
- APOBEC1-HA-P2A-TdTomato (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer RPS2
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_EF1a>RPS2-APOBEC1-p2a-TdTomato-WPRE249-BGHpA was a gift from Eugene Yeo (Addgene plasmid # 254839 ; http://n2t.net/addgene:254839 ; RRID:Addgene_254839) -
For your References section:
Single-cell and isoform-specific translational profiling of the mouse brain. Sison SL, Zampa F, Kofman ER, Choi SY, Jagannatha P, Nguyen GG, Naritomi JT, Shin A, Khorgade A, Jin W, Chen CY, Sievert DM, Mukhopadhyay S, Mizrahi O, Blue SM, Marina RJ, Yang D, Wang CC, Pang Z, Brannan KW, Ye L, Al'Khafaji AM, Yeo GW, Lippi G. Nature. 2026 Feb 18. doi: 10.1038/s41586-026-10118-1. 10.1038/s41586-026-10118-1 PubMed 41708856