pSEP4C
(Plasmid
#254855)
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Purpose(Empty Backbone) SpARC-map backbone with neoRN fragment fused to the C-terminus of the bait protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneCustom backbone
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Vector typeBacterial Expression, Synthetic Biology
- Promoter trc, pLtetO-1
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Tag
/ Fusion Protein
- neoR[1-59], neoR[59-264] (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEP4C was a gift from Stephen Benkovic (Addgene plasmid # 254855 ; http://n2t.net/addgene:254855 ; RRID:Addgene_254855) -
For your References section:
Multiplex mapping of protein-protein interaction interfaces. He J, Zou LN, Pareek V, Benkovic SJ. Proc Natl Acad Sci U S A. 2025 Dec 30;122(52):e2425774122. doi: 10.1073/pnas.2425774122. Epub 2025 Dec 22. 10.1073/pnas.2425774122 PubMed 41428874
