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pKV67
(Plasmid #254865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254865 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDG1730
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    LB at 37 ℃ supplemented with 100 µg/mL ampicillin. Select with spectinomycin 100 µg/mL for integration into the B. subtilis chromosome at amyE.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    tetracycline repressor
  • Alt name
    tetR(B)

Gene/Insert 2

  • Gene/Insert name
    mcherry

Gene/Insert 3

  • Gene/Insert name
    spectinomycin adenyltransferase
  • Alt name
    specR

Gene/Insert 4

  • Gene/Insert name
    erythromycin ribosomal methylase
  • Alt name
    erm

Gene/Insert 5

  • Gene/Insert name
    beta-lactamase
  • Alt name
    ampR

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.17.676849 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKV67 was a gift from Elizabeth Libby (Addgene plasmid # 254865 ; http://n2t.net/addgene:254865 ; RRID:Addgene_254865)

  • For your References section:

    A multi-tetracycline responsive induction system for gene expression in Bacillus subtilis. Reich-Veillette K, Rhoads AE, Libby EA. Appl Environ Microbiol. 2026 May 20;92(5):e0021026. doi: 10.1128/aem.00210-26. Epub 2026 Apr 23. 10.1128/aem.00210-26 PubMed 42024128
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