pUA66-PnagA reporter
(Plasmid
#254869)
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PurposeAn edited version of PnagA reporter from Zaslaver et al. promoter collection that includes RegulonDB-verified nagA promoter upstream of GFP.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUA66
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPmut2
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Insert Size (bp)836
- Promoter PnagA
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.27.625634 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUA66-PnagA reporter was a gift from Mary Dunlop (Addgene plasmid # 254869 ; http://n2t.net/addgene:254869 ; RRID:Addgene_254869) -
For your References section:
Dynamic heterogeneity in an E. coli stress response regulon mediates gene activation and antimicrobial peptide tolerance. Blassick CM, Moghimianavval H, Jafarbeglou F, Lugagne JB, Dunlop MJ. Cell Rep. 2026 Apr 16;45(4):117259. doi: 10.1016/j.celrep.2026.117259. 10.1016/j.celrep.2026.117259 PubMed 41996246
Map uploaded by the depositor.
